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Consumer Product Safety Commission (CPSC) (1980). Federal Hazardous Substances Act Regulations, CFR 150, 41. Dannenberg, A. , Moore, K. , Schofield, B. , Pula, P. , and Bassett, D. P. (1987). Two new in vitro methods for evaluating toxicity to skin (employing short-term organ culture). In Alternative Methods in Toxicology (A. M. ), Vol. 5, pp. 115-128. Liebert, New York. DeLeo, V. , Harber, L. , Kong, B. , and Salva, S. D. (1987). Surfactantinduced cutaneous primary irritancy: An in vitro model.

In Principles and Methods of Toxicology (A. W. ), pp. 209-222. Raven Press, New York. Gordon, V. , Kelly, C. , and Bergman, H. C. (1989). SKINTEX, an in vitro method for determining dermal irritation. International Congress of Toxicology, Brighton, UK. Gordon, V. , Kelly, C. , and Bergman, H. C. (1990). Evaluation of SKINTEX, an in vitro method for determining dermal irritation. Toxicologist 10(1), 78. Guillot, J. , Gonnet, J. , and Trahaut, R. (1982). Evaluation of the cutaneous-irritation potential of 56 compounds.

2. All rabbits selected for the study must be in good health; any rabbit exhibiting snuffles, hair loss, loose stools, or apparent weight loss is rejected and replaced. 3. One day (at least 18 hr) prior to application of the test substance, each rabbit is prepared by clipping the hair from the back and sides using a small animal clipper. A size No. 10 blade is used to remove the long hair and then a size No. 40 blade is used to remove the remaining hair. 37 3 Tests for Dermal Irritation and Corrosion PRIMARY DERMAL IRRITATION STUDY DESIGN Species: Strain" Test Group" Rabbit New Zealand White 6 Animals Initial Observation Period Additional Daily Observation If Irritation is Persistant .....

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